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broth microdilution methods for yeast  (Clinical and Laboratory Standards Institute)

 
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    Clinical and Laboratory Standards Institute broth microdilution methods for yeast
    Broth Microdilution Methods For Yeast, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/broth+microdilution+method+for+yeast/clsi+guidelines/us12269814-486-21-16
    Average 90 stars, based on 1 article reviews
    broth microdilution methods for yeast - by Bioz Stars, 2026-10
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    Article Title: Trichosporon asahii causing subcutaneous mycoses in an immunocompetent patient: case report and a minireview
    Article Snippet: Conventional biochemical tests identified the isolate as T. asahii , and the identification was confirmed in triplicate (2.2 score) by the MALDI-TOF MS technique using the Microflex LT spectrometer and Biotyper 3.3 software package (Bruker Daltonics, Billerica, MA, USA), according to the manufacturer’s recommendations.



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    Workflow and optimization. (A) Scheme of anti-Candida auris <t>susceptibility</t> assay. Candida auris harvested from colonies grown on Sabouraud dextrose agar (SDA) were incubated in RPMI medium with compounds to identify inhibitors. (B and C) Optimization of the ATP-based antifungal susceptibility assay by fungal density and incubation time. Cells were diluted with fresh medium into ratios of 1 to 1,000, 1 to 500, and 1 to 200 prior to dispensing into assay plates. (B) Fungal growth was detected by an ATP-based assay at 24 and 48 h. (C) Two known compounds, posaconazole and micafungin, were tested against strains 0384 and 0390 to serve as a positive control.
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    Workflow and optimization. (A) Scheme of anti-Candida auris susceptibility assay. Candida auris harvested from colonies grown on Sabouraud dextrose agar (SDA) were incubated in RPMI medium with compounds to identify inhibitors. (B and C) Optimization of the ATP-based antifungal susceptibility assay by fungal density and incubation time. Cells were diluted with fresh medium into ratios of 1 to 1,000, 1 to 500, and 1 to 200 prior to dispensing into assay plates. (B) Fungal growth was detected by an ATP-based assay at 24 and 48 h. (C) Two known compounds, posaconazole and micafungin, were tested against strains 0384 and 0390 to serve as a positive control.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Identification of Antifungal Compounds against Multidrug-Resistant Candida auris Utilizing a High-Throughput Drug-Repurposing Screen

    doi: 10.1128/AAC.01305-20

    Figure Lengend Snippet: Workflow and optimization. (A) Scheme of anti-Candida auris susceptibility assay. Candida auris harvested from colonies grown on Sabouraud dextrose agar (SDA) were incubated in RPMI medium with compounds to identify inhibitors. (B and C) Optimization of the ATP-based antifungal susceptibility assay by fungal density and incubation time. Cells were diluted with fresh medium into ratios of 1 to 1,000, 1 to 500, and 1 to 200 prior to dispensing into assay plates. (B) Fungal growth was detected by an ATP-based assay at 24 and 48 h. (C) Two known compounds, posaconazole and micafungin, were tested against strains 0384 and 0390 to serve as a positive control.

    Article Snippet: A high-throughput screening assay was first developed by an adaptation of a standard broth microdilution method for antifungal susceptibility testing of yeasts posted by the Clinical and Laboratory Standards Institute (CLSI) ( ) ( 18 ).

    Techniques: Drug Susceptibility Assay, Incubation, ATP Assay, Positive Control

    Antifungal susceptibility profile against flucytosine and myriocin combination

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Identification of Antifungal Compounds against Multidrug-Resistant Candida auris Utilizing a High-Throughput Drug-Repurposing Screen

    doi: 10.1128/AAC.01305-20

    Figure Lengend Snippet: Antifungal susceptibility profile against flucytosine and myriocin combination

    Article Snippet: A high-throughput screening assay was first developed by an adaptation of a standard broth microdilution method for antifungal susceptibility testing of yeasts posted by the Clinical and Laboratory Standards Institute (CLSI) ( ) ( 18 ).

    Techniques: